Affinity chromatography using agarose–triazine derivatives

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Abstract
The preparation of derivatives of s-triazines and agarose and their application to the purification of chymotrypsin (E.C. 3.4.4.5), lactate dehydrogenase (E.C. 1.1.1.27), riboflavin synthase (E.C. 2.5.1.9), and dihydropteroate synthase (E.C. 2.5.1.15) is described. Methods for the preparation of both amino- and carboxy-activated agaroses are discussed. The stability of the chromatographic adsorbents prepared with s-triazines to hydrolysis and storage is shown by radioactivity measurements to be substantially greater than that of comparable adsorbents; s-tri-azinylagarose derivatives lose less than 1% of ligand in 2 months' storage and are fully active after 2 years' storage.