SEPARATION OF CFU-S FROM PRIMITIVE CELLS RESPONSIBLE FOR RECONSTITUTION OF THE BONE-MARROW HEMATOPOIETIC STEM-CELL COMPARTMENT FOLLOWING IRRADIATION - EVIDENCE FOR A PRE-CFU-S CELL

  • 1 March 1989
    • journal article
    • research article
    • Vol. 17  (3) , 263-266
Abstract
We have studied the in vivo spleen colony-forming ability and marrow repopulating ability of murine bone marrow cells differing in mitochondrial activity. Following centrifugal elutriation the cells were sorted on the basis of rhodamine-123 fluorescence intensity within a predetermined light scatter window. It is shown that a class of hemopoietic stem cells exists that differs from the majority of day-12 spleen colony-forming units (CFU-S) in that it has low mitochondrial activity and a high capacity to generate in time new day-12 CFU-S and cells that rescue recipients from radiation-inflicted death. These data add direct evidence for the identity of pre-CFU-S and CFU-S by physical separation.