CD3+ T-cells in severe combined immunodeficiency (scid) mice III. Transferred congenic, selfreactive CD4+T cell clones rescue IgM-producing, Scid-derived B cells
- 1 July 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 3 (7) , 657-663
- https://doi.org/10.1093/intimm/3.7.657
Abstract
Young severe combined immunodeficiency (scid) mice completely lack immunocompetent lymphocytes. Limiting numbers of purified CD4+T cells from allotype-congenic BALB/c (lghb) donor mice were transplanted into 3-week-old scid (lghb) recipient mice. Splenic CD4+ T cells were recovered from transplanted scid mice 10–12 weeks post-transfer and established as T cell lines In culture. These T cell populations proliferated in vitro in response to syngeneic stimulator cells. T cell clones derived In vitro from these T cell lines displayed selfreactive recognition specificity: these CD4+ T cells proliferated in vitro In response to syngenelc/congenelc but not allogeneic stimulator cells. Cloned selfreactive T cells retransplanted Into young scid recipients were engrafted into spleens of secondary recipients, did not Induce autoimmune disease but stimulateddevelopment of scld-derived (lghb), IgM-producing B cells (B cell leakiness).Keywords
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