STUDY OF HUMAN T AND B LYMPHOCYTES WITH HETEROLOGOUS ANTISERA .2. PARTIAL CHARACTERIZATION OF A HUMAN T LYMPHOCYTE ANTIGEN

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 31  (5) , 759-765
Abstract
Using a horse anti-human thymocyte serum made specific for T [thymus-derived] lymphocytes by absorption (anti-HTLA [human T lymphocyte antigen] serum), a material reacting with it was extracted from detergent-lysed peripheral blood lymphocytes. Acid elution ang gel-filtration analysis allowed recovery of 2 peaks containing HTLA activity: one of 30,000-40,000 daltons, the other being excluded by Sephadex G-200 and likely to be more than 300,000 daltons. HTLA appeared to be released by lymphocytes incubated at 37.degree. but not at 4.degree., and significantly detectable after 10-24 h incubation. Co-capping experiments with anti-.beta.2m [.beta.2 microglobulin] antibodies and absence of inhibition of cytolysis by anti-HTLA serum of cells coated with anti-.beta.2m F(ab)''2 fragments excluded association of HTLA and .beta.2m.