INDIUM-111-LABELED LDL - A POTENTIAL AGENT FOR IMAGING ATHEROSCLEROTIC DISEASE AND LIPOPROTEIN BIODISTRIBUTION
- 1 March 1990
- journal article
- research article
- Vol. 31 (3) , 343-350
Abstract
Radiolabeling of low-density lipoprotein (LDL) and external imaging with a gamma camera would offer a means of taking advantage of the metabolic activity of developing atherosclerotic lesions in order to noninvasively detect and determine the extent of atherosclerotic cardiovascular disease. Indium-111-(111In) labeled LDL was prepared and its purity demonstrated by agarose electrophoresis and ultracentrifugation. In vitro studies with cultured human fibroblasts demonstrated significant inhibition of iodine-125-(125I) LDL binding to LDL receptors by 111In-LDL, although this was less than the inhibition produced by unlabeled LDL. Adrenal gland uptake of 111In-LDL by hypercholesterolemic rabbits was reduced by 86% compared to the level of uptake observed in normal rabbits. These results were compatible with downregulation of adrenal LDL receptors in the hypercholesterolemic rabbits. Uptake of 111In-LDL in the atherosclerotic proximal aorta of hypercholesterolemic rabbits was 2.5 times higher than in normal rabbits. These results suggest that 111In-LDL has the potential to be a useful agent for external imaging of atherosclerotic lesions and lipoprotein biodistribution.This publication has 16 references indexed in Scilit:
- The preparation of DTPA-coupled antibodies radiolabeled with metallic radionuclides: an improved methodJournal of Immunological Methods, 1983
- A radioiodinated, intracellularly trapped ligand for determining the sites of plasma protein degradation in vivoBiochemical Journal, 1983
- EVALUATION OF THE VIABILITY OF IN-111-LABELED DTPA COUPLED TO FIBRINOGEN1982
- Saturation and suppression of hepatic lipoprotein receptors: a mechanism for the hypercholesterolemia of cholesterol-fed rabbits.Proceedings of the National Academy of Sciences, 1981
- Low Density Lipoprotein Receptors in Bovine Adrenal Cortex. II. Low Density Lipoprotein Binding to Membranes Prepared from Fresh Tissue*Endocrinology, 1979
- Role of lysine residues of plasma lipoproteins in high affinity binding to cell surface receptors on human fibroblasts.Journal of Biological Chemistry, 1978
- The distribution of labeled low-density lipoproteins across the rabbit thoracic aorta in vivoAtherosclerosis, 1977
- Effect of hypertension on the entry of 125I-labelled low density lipoprotein into the aortic intima in normal-fed rabbitsAtherosclerosis, 1976
- Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycansCell, 1976
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951