Purification of Eimeria Sporozoites by DE‐52 Anion Exchange Chromatography
- 1 February 1984
- journal article
- Published by Wiley in The Journal of Protozoology
- Vol. 31 (1) , 181-183
- https://doi.org/10.1111/j.1550-7408.1984.tb04314.x
Abstract
An anion exchange column of DE-52 has been used to purify Eimeria sporozoites from a post- excystation mixture of oocysts, oocyst shells, sporocysts, sporocyst shells, and sporozoites. The mean recovery from several experiments was 94% and virtually all non-sporozoite material was removed. Infectivity studies in vitro with sporozoites showed that they were viable after purification and were at least as infectious as the unpurified sporozoites; furthermore, oocysts in the crude preparation could be recovered from the DE-52 cellulose by resuspending them in a 20% (w/v) sodium chloride solution.Keywords
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