Multiresidue Method for Confirmation of Macrolide Antibiotics in Bovine Muscle by Liquid Chromatography/Mass Spectrometry
Open Access
- 1 March 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 79 (2) , 397-404
- https://doi.org/10.1093/jaoac/79.2.397
Abstract
A particle beam/liquid chromatographic/mass spectrometric (PB/LC/MS) method capable of determining 5 macrolides in bovine muscle is described. Spiramycin, tylosin, tilmicosin, erythromycin, and josamycin residues in bovine muscle are confirmed by reversed-phase LC/MS incorporating gradient elution. Macrolides are extracted from tissue with chloroform, and the extract is purified with a diol solid-phase extraction cleanup. The 5 macrolides are identified by negative and positive chemical ionization with selective ion monitoring of 2 ions in each mode. The procedure confirms the presence of each macrolide at 50 μg/kg in spiked samples.Keywords
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