Tricarboxylic acid-cycle activity in Streptomyces olivaceus

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Abstract
Some evidence has been presented for the occurrence of the tricarboxylic acid cycle in Streptomyces olivaceus. The intermediates of this cycle stimulate oxygen uptake over the endogenous respiration by the whole resting cells. The cell-free extracts contained the following enzymes: aconitase, isocitric dehydrogenase, succinic dehydrogenase, malic dehydrogenase, glutamic dehydrogenase, alanine dehydrogenase, reduced diphosphopyridine nucleotide oxidase, reduced triphosphopyridine nucleotide-cytochrome c reductase and reduced diphosphopyridine nucleotide cytochrome c reductase; the extracts were capable of synthesizing citrate from acetate, coenzyme A, adenosine triphosphate, inorganic phosphate and oxaloacetate. Activities of pyruvic and [alpha]-oxoglutaric dehydrogenases, fumarase and cytochrome c oxidase could not be demonstrated in the cell-free extracts. Cells grown on acetate possess isocitratase and malate synthetase.