Multiple forms of sarc gene proteins from Rous sarcoma virus RNA.

Publisher Website
Google Scholar
Abstract
In a previous study 2 proteins of 25,000 and 18,000 daltons that were made from RNA of transforming virions of Rous sarcoma virus (RSV) and that were missing from the translation products of a transformation-defective deletion mutant of RSV were identified. In the present study RSV virion RNA was separated on sucrose gradients and determined that the 2 putative sarc gene products are synthesized as doublets from an mRNA of approximately 18S. There also appear to be several other sizes of virion mRNA that direct the synthesis of other viral proteins. These data are discussed in terms of the structure of the RSV genome. Besides the 25,000 and 18,000 dalton doublets, there also is a 60,000 dalton protein whose synthesis is directed by 18S viral RNA from transforming virion of RSV. Peptide mapping showed that the 60,000 and the 25,000 dalton doublet are structurally related. The use of 2-dimensional gel electrophoresis resolved both bands of the 25,000 dalton doublet into several differently charged species.