A Plant Receptor-Like Gene, the S-Locus Receptor Kinase of Brassica oleracea L., Encodes a Functional Serine/Threonine Kinase
Open Access
- 1 March 1993
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 101 (3) , 1103-1106
- https://doi.org/10.1104/pp.101.3.1103
Abstract
To investigate the catalytic properties of the Brassica oleracea S-locus receptor kinase (SRK), we have expressed the domain that is homologous to protein kinases as a fusion protein in Escherichia coli. Following in vivo labeling of cultures with 32P-labeled inorganic phosphate, we observed phosphorylation of the fusion protein on serine and threonine, but not on tyrosine. In contrast, labeling was not observed when lysine-524, a residue conserved among all protein kinases, was mutated to arginine, thus confirming that SRK phosphorylation was the result of intrinsic serine/threonine kinase activity.Keywords
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