Studies on the Replication of a Satellite RNA Associated with Cymbidium Ringspot Virus

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Abstract
The replication of cymbidium ringspot tombusvirus satellite RNA was investigated Nicotiana clevelandii plants. The temperature at which host plants were grown strongly influenced the synthesis of the satellite RNA; high temperatures were detrimental to, or even inhibited, its multiplication. The most favourable temperature range was 16 to 25.degree. C. The termini of the satellite RNA were studied and a free hydroxyl group was found at the 3'' but not at the 5'' end, which may explain why circular forms were not detected in infected tissue. On the other hand, multimers of double-stranded RNA were present, which may have arisen independently of the formation of circular molecules.