Multiresidue Recovery at PPB Levels of 10 Nitrosamines from Frankfurters by Supercritical Fluid Extraction

Abstract
The design of a laboratory-assembled supercritical fluid extractor is described for the efficient recovery of volatile nitrosamines from a common-cured meat product, frankfurters. The principal feature of the apparatus was a newly designed restrictor—collector interface where a commercial solid-phase extraction cartridge was directly attached to the micrometering valve. This reduced the path length between the discharge tube and the 1 g silica gel sorbent bed. The elapsed time for each 2.5 g sample extraction with supercritical CO2 was 17 min. The nitrosamines were separated and detected using a gas-chromatographic chemiluminescence (Thermal Energy Analyzer, Thermedics, Inc.; Woburn, MA) system. Recovery of 10 volatile aliphatic and alicyclic nitrosamines from frankfurters, fortified at the 20 ppb level, ranged from 84.3 to 104.8% with relative standard deviation of 2.34 to 6.13%.

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