GnRH RECEPTORS IN HUMAN GRANULOSA CELLS: ANATOMICAL LOCALIZATION AND CHARACTERIZATION BY AUTORADIOGRAPHIC STUDY
- 1 September 1989
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 125 (3) , 1739-1741
- https://doi.org/10.1210/endo-125-3-1739
Abstract
The presence of receptors for GnRH in human ovary has been investigated by quantitative autoradiography. Simultaneous visualization and characterization of specific receptors on frozen sections were obtained on six pairs of human ovaries. Among them only one exhibited a large preovulatory follicle. This dominant follicle exhibited a specific and high affinity binding capacity for 125I-GnRHa exclusively localized on the granulosa cell layer. Analysis of saturation curve indicates a Kd value of 0.22 nM and Bmax of 9.6 fmol/mg protein. In contrast LH-hCG binding sites were present in all antral follicles. These data demonstrate for the first time the presence of high affinity GnRH receptors in human granulosa cells at the late stage of follicular maturation.This publication has 10 references indexed in Scilit:
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