Identification of the Cloned Gene for the Murine Transplantation Antigen H-2Kbby Hybridization with Synthetic Oligonucleotides
Open Access
- 1 April 1983
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 3 (4) , 750-755
- https://doi.org/10.1128/mcb.3.4.750
Abstract
The H-2Kbgene is a member of the large major histocompatibility complex class I gene family. Since many members of this family cross-hybridize with class I cDNA probes, the cloned H-2Kbgene was identified by hybridization with specific oligonucleotide probes. This clone was definitively shown to encode the H-2Kbpolypeptide by partial DNA sequencing and by serological and tryptic peptide analyses of the expressed product.Keywords
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