Insertion of a Coiled-Coil Peptide from Influenza Virus Hemagglutinin into Membranes
- 14 October 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 266 (5183) , 274-276
- https://doi.org/10.1126/science.7939662
Abstract
The trimeric protein hemagglutinin (HA) of the influenza viral envelope is essential for cell entry. To investigate the interaction of HA with membranes, two 40-residue, cysteine-substituted peptides comprising the loop region and the first part of the coiled-coil stem were synthesized and modified with a nitroxide spin label. Electron paramagnetic resonance analysis revealed that the peptide inserts reversibly into phospholipid vesicles under endosomal pH conditions. This result suggests that some or all of the long coiled-coil trimer of HA may insert into membranes, which could bring the viral and cell membranes closer together and facilitate fusion.Keywords
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