Immortalization of epithelial cells
- 1 January 1996
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 270 (1) , C1-C11
- https://doi.org/10.1152/ajpcell.1996.270.1.c1
Abstract
The methodologies for isolating cell lines have become very powerful, particularly in terms of retaining differentiated features of the parent cells. Cell lines can be developed from primary or early passage cells as well as from transgenic animals that carry an immortalizing gene. Cell lines from epithelia have been selected for their polar orientation, tight junction formation, and expression of differentiated markers or functions. These cell lines provide useful models for studying cell biology of specific tissues, tumorigenicity, genetic abnormalities, or to help screen for effective methods of gene therapy.Keywords
This publication has 32 references indexed in Scilit:
- Retinoid regulation of cell differentiation in a series of human papillomavirus type 16-immortalized human cervical epithelial cell linesCarcinogenesis: Integrative Cancer Research, 1995
- Immortalization of immature and mature mouse astrocytes with SV40 T antigenJournal of Neuroscience Research, 1994
- Regulation of insulin‐like growth factor 1 binding protein 3 levels by epidermal growth factor and retinoic acid in cervical epithelial cellsJournal of Cellular Physiology, 1994
- CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells.American Journal of Respiratory Cell and Molecular Biology, 1994
- Calcium Regulates the Differentiation of Human Papillomavirus Type 16 (HPV16) Immortalized Ectocervical Epithelial Cells, but Not the Expression of the Papillomavirus E6 and E7 OncogenesExperimental Cell Research, 1993
- A transformed human epithelial cell line that retains tight junctions post crisisIn Vitro Cellular & Developmental Biology – Animal, 1992
- Characterization of immortal cystic fibrosis tracheobronchial gland epithelial cells.Proceedings of the National Academy of Sciences, 1992
- Chloride Ion Transport in Transformed Normal and Cystic Fibrosis Epithelial CellsPublished by Springer Nature ,1991
- Structure, function, and differentiation of the keratinocytePhysiological Reviews, 1989
- An N-Terminal transformation-governing sequence of SV40 large T antigen contributes to the binding of both p110 and a second cellular protein, p120Cell, 1989