Light-dependent subcellular localization of nucleoside diphosphate kinase-1 inNeurospora crassa
Open Access
- 1 August 2006
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 261 (1) , 64-68
- https://doi.org/10.1111/j.1574-6968.2006.00329.x
Abstract
Nucleoside diphosphate kinase (NDK) is a housekeeping enzyme localized in cellular organelles and distributed in various organs in prokaryotes and eukaryotes. In Neurospora crassa, NDK-1 is suggested to control catalases in response to heat, oxidative stress and light. In this study, we identified the presence of NDK-1 during most developmental stages in submerged mycelia, aerial hyphae, asexual conidia and perithecia, and the localization of it in soluble, mitochondrial, nuclear and membrane fractions in the mycelial cell. A light-dependent localization of NDK-1 was shown by Western blotting and immunohistochemical analysis using anti-NDK-1 antibody. In the mycelia, NDK-1 was compartmentalized on the plasma membrane in darkness, while it was relocated in the cytoplasm under light. These results suggest that NDK-1 protein was translocated from the plasma membrane to cytoplasm in response to light, and may interact with catalase.Keywords
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