QUANTITATIVE SURVIVAL OF TOXOPLASMA-GONDII TACHYZOITES AND BRADYZOITES IN PEPSIN AND IN TRYPSIN SOLUTIONS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 42  (1) , 128-130
Abstract
Bradyzoites of T. gondii survived in pepsin solution and in trypsin solution. After 120 min in trypsin solution at 37.degree. C, infectivity of bradyzoites for mice was reduced 100-fold; in pepsin solution there was no reduction in infectivity. Tachyzoites survived in trypsin but after 15 and 60 min. of incubation at 37.degree. C, there was a 100,000-fold reduction in infectivity titer. T. gondii was demonstrated in mice inoculated with experimentally infected goat brain digested in pepsin and in trypsin solutions but not in mice inoculated with undigested brain of the same goat. Tissues should be digested in pepsin solution to isolate T. gondii from chronically infected animals.

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