Phytochrome Immunoaffinity Purification
- 1 August 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 64 (2) , 332-336
- https://doi.org/10.1104/pp.64.2.332
Abstract
A phytochrome immunoaffinity purification procedure was developed that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of > 98% purity within 2 h when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The immunoaffinity technique was also used to purify phytochrome from crude oat extracts, from brushite-purified pea (Pisum sativum L., cv. Alaska) and rye (Secale cereale L., cv. Balbo) preparations.This publication has 15 references indexed in Scilit:
- Phytochrome RadioimmunoassayPlant Physiology, 1979
- SPECTRAL CHARACTERIZATION OF HIGH‐MOLECULAR‐WEIGHT PHYTOCHROME*Photochemistry and Photobiology, 1975
- Immunological and Physical Characterization of the Products of Phytochrome ProteolysisPlant Physiology, 1975
- An immunochemical characterization of the phytochrome destruction reactionBiochimica et Biophysica Acta (BBA) - Protein Structure, 1974
- Purification of Oat and Rye PhytochromePlant Physiology, 1973
- Comparative Immunochemistry of PhytochromePlant Physiology, 1973
- Cyclic nucleotide phosphodiesterase in pea seedlingsBiochimica et Biophysica Acta (BBA) - Enzymology, 1972
- “Disaggregation” of Phytochrome in Vitro—A Consequence of ProteolysisPlant Physiology, 1971
- Immunocytochemical Localization of PhytochromeProceedings of the National Academy of Sciences, 1971
- The Aggregation States of Phytochrome from Etiolated Rye and Oat SeedingsPlant Physiology, 1970