Determination of Lactate Dehydrogenase Isoenzyme (LD-1) Using Flow Injection Analysis with Electrochemical Detection after Immunochemical Separation

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Abstract
A flow system has been developed for a fast and convenient assay of LD-1 and total LD in serum. The LD-1 is separated immunochemically and subsequently determined amperometrically by a thin layer transducer in a flow system. Potassium ferricyanide was used as a mediator at a potential of 0.07 V vs Ag/AgCl to eliminate any protein fouling of the electrode. A kinetic measurement helps to diminish several interferences in the electrode response. Thus, it is possible to determine the LD-1 or LD-1/total LD conveniently and accurately even at low activities.