SPECIFIC BINDING SITES FOR L-TRIIODOTHYRONINE IN RAT LIVER AND KIDNEY CYTOSOL
- 1 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 82 (1) , 98-104
- https://doi.org/10.1530/acta.0.0820098
Abstract
Binding of 125I-labeled triiodothyronine (T3) to rat liver and kidney cytosol was examined. In both organs a very similar binding protein was detected. The apparent equilibrium association constants amounted to 4.8 .times. 107 M-1 for the liver protein and to 1.8 .times. 107 M-1 for the kidney protein. In the 2 organs a high capacity for T3 binding was observed; maximal binding capacities of, respectively, 4.3 and 33.2 pmol/mg total cytosol protein. Displacement studies using thyroid hormone analogues showed that the binding proteins exhibited similar specificity.This publication has 1 reference indexed in Scilit:
- A graphic method for the determination and presentation of binding parameters in a complex systemAnalytical Biochemistry, 1967