Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis.
Open Access
- 1 September 1995
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 182 (3) , 865-874
- https://doi.org/10.1084/jem.182.3.865
Abstract
We have identified a serine/threonine kinase that is rapidly activated during Fas-mediated apoptosis. Fas-activated serine/threonine kinase (FAST) is phosphorylated on serine and threonine residues in Jurkat cells. In response to Fas ligation, it is rapidly dephosphorylated and concomitantly activated to phosphorylate TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Phosphorylation of TIA-1 precedes the onset of DNA fragmentation, suggesting a role in signaling downstream events in the apoptotic program. Our results introduce Fast and TIA-1 as components of a molecular cascade involved in signaling Fas-mediated apoptosis.Keywords
This publication has 44 references indexed in Scilit:
- Cytotoxic effector cell granules recognized by the monoclonal antibody TIA-1 are present in CD8+ lymphocytes in lymph nodes of human immunodeficiency virus-1-infected patients.1993
- Differential activation of antigen-stimulated suicide and cytokine production pathways in CD4+ T cells is regulated by the antigen-presenting cell.The Journal of Immunology, 1993
- Identification of a Ten-Amino Acid Proline-Rich SH3 Binding SiteScience, 1993
- ApoptosisImmunology Today, 1993
- Differential expression of apoptosis-related Fas antigen on lymphocyte subpopulations in human peripheral blood.The Journal of Immunology, 1992
- Identification and functional characterization of a TIA-1-related nucleolysin.Proceedings of the National Academy of Sciences, 1992
- Evidence of cytotoxic T-cell destruction of epidermal cells in human graft-vs-host disease. Immunohistology with monoclonal antibody TIA-1.1992
- Expression of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) is required for virus growth and neoplastic transformationJournal of General Virology, 1992
- Structural similarity between Fc receptors and T cell receptors. Expression of the gamma-subunit of Fc epsilon RI in human T cells, natural killer cells and thymocytes.The Journal of Immunology, 1991
- Characterization of a ceramide-activated protein kinase: stimulation by tumor necrosis factor alpha.Proceedings of the National Academy of Sciences, 1991