Extended in vitro selection for synthesis of novel molecular recognition oligonucleotide derivatives
Open Access
- 1 November 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Symposium Series
- Vol. 42 (1) , 177-178
- https://doi.org/10.1093/nass/42.1.177
Abstract
In vitro selection of RNA aptamer containing biotin-carrying nucleotide was carried out used for development of a new type of molecular sensor. Cytidine 5′-triphosphate (CTP) carrying biotinyl group at the N6-position was used in this technique. A pool of biotin-containing RNAs, which binds specifically to adenosine 5′-triphosphate (ATP), was obtained and used for competitive binding assay of ATP. The selected nonnatural RNA possesses many biotinyl groups to render it a high sensitivity toward ATP.Keywords
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