Sexual Response of Saccharomyces cerevisiae: Phosphorylation of Yeast Glyoxalase I by a Cell Extract of Mating Factor-Treated Cells

Abstract
The phosphorylation of glyoxalase I was observed when the phosphatase-treated enzyme was incubated in the presence of [γ-−32P] ATP and a cell extract prepared from α-type yeast cells which had been treated with the culture supernatant of a-type yeast cells. The phosphorylated protein was identified as glyoxalase I by using anti-glyoxalase I rabbit immunoglobulin G.

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