Séparation chromatographique par des gels de polystyrène preparés par réticulation anionique

Publisher Website
Google Scholar
Abstract
Chromatographic columns were filled with anionically prepared polystyrene gels of various porosities. Linear polystyrene samples were injected in these columns and the retention volumes were measured. Normalized curves have been compared, the dynamic partition coefficient being plotted versus the logarithm of the molecular weight of the injected polymer. It has been established that the domain of molecular weights in which chromatographic separation may take place depends upon the parameters characterizing the gel: molecular weight of the linear “precursor”, overall concentration at the gel point, proportion of crosslinking agent.It has been found that the chromatographic retention times depend on such factors as size of gel particles, flow‐rate of the elution solvent. This shows that the exchange equilibrium between the pores of the gels and the flowing intersticial liquid are far from being fulfilled. However by choosing standard conditions one gets reproducible results which may be adequately compared.It should be emphasized that macroporosity of a gel is not a prerequisit for chromatographic separation.

This publication has 3 references indexed in Scilit: