HYBRIDIZATION BETWEEN LYMPHOMA-T AND LYMPHOMA-B CELL-LINES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 39  (1) , 57-60
Abstract
The AKR thymoma line BW 5147 was successfully hybridized with the Ig[immunoglobulin]M-bearing (BALB/c .times. NZB)F1 B [bone marrow-derived] lymphoma line WEHI 231. In the hybrids formed, T[thymus-derived]-cell characteristics were dominant, i.e., there was no expression of IgM but continued expression of Thy-1.1 in 8 of 8 lines. In 2 of 8 lines, the Thy-1.2 allele was also expressed. In its ability to hybridize, BW 5147 is not restricted to cells to similar ontogenetic origin (i.e., T cells) and fusion with the thymoma can lead to suppression of B cell gene expression and derepression of genes for T cell markers.