A LINEAR PLASMID-LIKE DNA IN STREPTOMYCES SP. PRODUCING LANKACIDIN GROUP ANTIBIOTICS
- 1 January 1979
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 25 (4) , 255-260
- https://doi.org/10.2323/jgam.25.255
Abstract
A linear plasmid-like DNA was isolated by Agarose gel electrophoresis from a lysate of Streptomyces sp. 7434-AN4 which produces lankacidin group antibodics. The DNA (pSLA2) with MW of 11.2 .times. 106 was cleaved into 5 and 3 fragments, respectively, with XmaI and BamNI on the definite sites from the end, but not digested by EcoRI and HindIII. Upon treatment of the strain with ethidium bromide, variants were obtained which have lost the ability to produce the antibiotics. These variants lost pSLA2. The linear plasmid-like DNA is apparently involved in the production of lankacidin group antibiotics.This publication has 4 references indexed in Scilit:
- The possible involvement of a plasmid(s) in actinomycin synthesis by Streptomyces parvulus and Streptomyces antibioticus.The Journal of Antibiotics, 1978
- Possible involvement of plasmids in biosynthesis of neomycin.The Journal of Antibiotics, 1978
- Isolation and characterization of four plasmids from Bacillus subtilisJournal of Bacteriology, 1977
- Genetic Determination of Methylenomycin Synthesis by the SCP1 Plasmid of Streptomyces coelicolor A3(2)Microbiology, 1977