Possible involvement of protein kinase C activation in differentiation of human umbilical vein endothelium‐derived cell into smooth muscle‐like cell

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Abstract
Summry— We previously reported that when deprived of fibroblast growth factor, human umbilical vein endothelium‐derived cells (HUVE‐DCs) are capable of differentiating into smooth muscle‐like cells through activin A‐induced, Smad‐dependent signaling, and that maintenance of the endothelial‐cell phenotype and differentiation into smooth muscle‐like cells are reciprocally controlled by fibroblast growth factor‐1 and activin A (Ishisaki et al., 2003). Here, we examined how protein kinase C (PKC), which plays pivotal roles in the regulation of cellular proliferation and differentiation in numerous cell types, might affect the above differentiation. We found that phorbol‐12‐myristate‐13‐acetate‐induced down‐regulations of some PKCs accompany suppressions of the expressions of smooth muscle cell markers in HUVE‐DCs deprived of fibroblast growth factor. Moreover, the PKC‐inhibitors Go¨6850 and Go¨6983 suppressed the differentiation of HUVE‐DCs into smooth muscle‐like cells. These results strongly suggest that activation of PKC is involved in the above differentiation.

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