Fluorescent Probes in the Study of the Extended Sites of Proteinases
- 1 January 1974
- journal article
- research article
- Published by Wiley in Israel Journal of Chemistry
- Vol. 12 (1-2) , 505-513
- https://doi.org/10.1002/ijch.197400040
Abstract
Studies on the kinetics of the cleavage of oligopeptide substrates by pepsin, together with the examination of the changes in fluorescence of dansyl or mansyl peptides upon interaction with pepsin or pepsinogen, give further evidence of the importance of secondary interactions of such substrates with the extended active site of the enzyme and indicate considerable conformational flexibility at the active site of pepsin in response to enzyme–substrate interaction.Keywords
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