Radioligand Binding and Inotropic Effects of Ryanodine in the Cardiomyopathic Syrian Hamster

Abstract
We compared the radioligand-binding and inotropic effects of ryanodine [known specific regulator of sarcoplasmic reticulum (SR) calcium release channel] on BIO 14.6 cardiomyopathic Syrian hamsters with those on age-matched F, B controls. Scatchard analyses of [3H]ryanodine binding to cardiac membranes prepared from 1–2-month-old BIO 14.6 and F1,B Syrian hamsters revealed the presence of a significant increase in binding capacity (Bmax = 942 ± 49 vs. 567 ± 33 fmol/mg protein) with no difference in affinity (KD = 3.9 ± 0.4 vs. 3.5 ± 0.5 nM, p

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