Flow Cytometry: A New Method To Investigate the Properties of Water-in-Oil-in-Water Emulsions
- 17 February 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 20 (6) , 2081-2085
- https://doi.org/10.1021/la035402+
Abstract
We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets “marked” with a fluorescent marker or “blank” without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC−BSA were investigated by this new method. The release fraction (after 24 h) of FITC−BSA from the w/o/w emulsion decreased with increasing concentration of FITC−BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.Keywords
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