On-line photon correlation spectroscopy using fibre-optic probes

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Abstract
The authors describe the design and construction of a fibre-optic probe for on-line photon correlation spectroscopy measurements. Two such probes, one acting as a launch and the other collecting scattered light, have been used to measure the size of proteins in the eluant of a gel permeation liquid chromatography column. The fibre-optic probe, when compared with conventional bulk optics, has the advantage of small size, ease of alignment and remote operation.