Aluminum lons stimulate DNA synthesis in quiescent cultures of Swiss 3T3 and 3T6 cells

Publisher Website
Google Scholar
Abstract
Micromolar concentrations of Al3+ are shown to be strongly mitogenic for quiescent cultures of Swiss 3T3 and 3T6 cells. Al3+ caused a striking shift in the dose-response curve for the effect of fetal bovine serum on 3H-thymidine incorporation. In the absence of serum the mitogenic effect of aluminum was greatly potentiated by insulin or cholera toxin, but not epidermal growth factor or 12–0-tetradecanoyl-phorbol-13-acetate. The stimulation of DNA synthesis was maximal by 15–20 μMM Al3+. Al3+ at 100 μMM had no inhibitory effect on DNA synthesis. Al3+ had no significant effect on cellular cyclic adenosine monophosphate in the presence or absence of insulin or an inhibitor of cyclic nucleotide phosphodiesterases.