Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-Molecular Complexes
- 7 May 2009
- book chapter
- Published by Springer Nature
- Vol. 544, 307-323
- https://doi.org/10.1007/978-1-59745-483-4_20
Abstract
Structural molecular biology over the past several decades has progressed from studies of the individual proteins, subunits, and domains that accomplish specific biochemistry to seeking to understand the dynamic bio-molecular complexes and assemblies that are responsible for biological function. This progress has led to an expansion of the structural analysis “tool box” to include methods that complement the mainstay techniques of the field: X-ray crystallography, nuclear magnetic resonance (NMR), and cryo-electron microscopy. Small-angle scattering of X-rays or neutrons is one such complementary technique that provides information on the size and shape of scattering particles in solution. This low-resolution structural information can be a powerful complement to high-resolution structural data, especially for the study of bio-molecular interactions with ligands or each other. Further, exploitation of the different neutron-scattering properties of the stable isotopes of hydrogen (^1H and ^2H) can be used to enrich the information available from the small-angle scattering data, especially for bio-molecular complexes.Keywords
This publication has 22 references indexed in Scilit:
- Joint use of small-angle X-ray and neutron scattering to study biological macromolecules in solutionEuropean Biophysics Journal, 2006
- Global Rigid Body Modeling of Macromolecular Complexes against Small-Angle Scattering DataBiophysical Journal, 2005
- Small-angle scattering studies of biological macromolecules in solutionReports on Progress in Physics, 2003
- Solving the generalized indirect Fourier transformation (GIFT) by Boltzmann simplex simulated annealing (BSSA)Journal of Applied Crystallography, 2000
- Restoring Low Resolution Structure of Biological Macromolecules from Solution Scattering Using Simulated AnnealingBiophysical Journal, 1999
- Low-Resolution Structures of Proteins in Solution Retrieved from X-Ray Scattering with a Genetic AlgorithmBiophysical Journal, 1998
- GNOM – a program package for small-angle scattering data processingJournal of Applied Crystallography, 1991
- Comparison of neutron and X-ray scattering of dilute myoglobin solutionsJournal of Molecular Biology, 1975
- Asymmetry in the 50S Ribosomal Subunit of Escherichia coliProceedings of the National Academy of Sciences, 1974
- A new method of studying the structure of block copolymers in solutionJournal of Polymer Science Part C: Polymer Letters, 1973