Sensitive Detection of Mycoplasma pneumoniae in Human Respiratory Tract Samples by Optimized Real-Time PCR Approach
- 1 August 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (8) , 2726-2730
- https://doi.org/10.1128/jcm.00321-07
Abstract
To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae , we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.Keywords
This publication has 19 references indexed in Scilit:
- Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical SamplesJournal of Clinical Microbiology, 2006
- Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing controlJournal of Medical Microbiology, 2006
- Detection of Mycoplasma pneumoniae in serum specimens from patients with mycoplasma pneumonia by PCRInternational Journal of Medical Microbiology, 2005
- Chlamydia pneumoniae and Mycoplasma pneumoniae in young children from China with community-acquired pneumoniaDiagnostic Microbiology and Infectious Disease, 2005
- Mycoplasma pneumoniaeand Its Role as a Human PathogenClinical Microbiology Reviews, 2004
- Molecular Diagnosis of Mycoplasma pneumoniae Respiratory Tract InfectionsJournal of Clinical Microbiology, 2003
- Subtyping of Mycoplasma pneumoniae isolates based on extended genome sequencing and on expression profilesInternational Journal of Medical Microbiology, 2003
- Detection ofMycoplasma pneumoniaeDNA in Cerebrospinal Fluid of a Patient withM. pneumoniaeInfection–“Associated” StrokeClinical Infectious Diseases, 2001
- Mycoplasma pneumoniae P1 Type 1- and Type 2-Specific Sequences within the P1 Cytadhesin Gene of Individual StrainsInfection and Immunity, 2001
- Mycoplasma pneumoniae infectionsCurrent Opinion in Infectious Diseases, 2001