Energetics of Cellular Repair Processes in a Respiratory-Deficient Mutant of Yeast

Publisher Website
Google Scholar
Abstract
Repair of potentially lethal damage induced by cytotoxic agents like UV irradiation (254 nm), psoralen-plus-UVA (365 nm) and methyl methanesulfonate was studied in the presence of a glucose analog, 2-deoxy-D-glucose, in yeast cells. Simultaneously, effects of 2-deoxy-D-glucose were also investigated on parameters of energy metabolism like glucose utilization, rate of ATP production, and ATP content of cells. 2-Deoxy-D-glucose is able to inhibit repair of potentially lethal damage induced by all the cytotoxic agents tested. The 2-deoxy-D-glucose-induced inhibition of repair depends upon the type of lesion and the pattern of cellular energy metabolism, the inhibition being greater in respiratory-deficient mutants than in the wild type. A continuous energy flow is necessary for repair of potentially lethal damage in yeast cells. Energy may be supplied by the glycolytic and/or the respiratory pathway; respiratory metabolism is not essential for this purpose. The magnitude of repair correlates with the rate of ATP production in a sigmoid manner.