Short-Term Kinetics of the Humoral Anti-Clq Response in SLE Using the ELISPOT Method: Fast Decline in Production in Response to Steroids

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Abstract
Twenty four systemic lupus erythaematosus patients and 17 patients with other diagnoses were investigated regarding the presence of cells producing Clq reactive antibodies in peripheral blood mononuclear cells using the ELISPOT technique. These results were then compared with parallel serum levels of Clq reactive antibodies. Current production of anti‐CIq was almost entirely confined to the systemic lupus erythaematosus group. Longitudinal analysis of anti‐Clq ELISPOT positive patients showed rapid changes in the number of anti‐Clq producing cells, but only slowly changing serum levels of the corresponding antibodies in response to glucocorticoids. In one systemic lupus erythaematosus patient prednisolone treatment had a selective effect on this autoantibody production, as the production of anti‐Clq spot forming cells rapidly dropped to zero, at the same time as the number of total spotforming cells showed only less change. In another patient, self‐limiting connective tissue disease was associated with temporal occurrence of IgM anti‐Clq. We believe, from these data, that the ELISPOT method for determination of current antibody production may be of particular value in longitudinal evaluation of disease course and therapeutic effects in systemic lupus erythaematosus and other rheumatic diseases.