Interconversion of 16-epioestriol and oestriol by avian liver tissue in vitro

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Abstract
[16-C14]16-Epiestriol and[6,7-3H2]estriol were incubated bothti simultaneously and separately at 40[degree] with hen liver homogenates obtained from birds in the laying stage. Purification and identification of products was made by ether extraction, Girard separation, Celite partition and paper chromatography and crystallization to constant specific activity both with and without derivative formation. Percentage conversions of the radioactive 16-epiestriol and estriol were calculatedd from these specific activities. Estriol yielded 16-epiestriol as early as 5 min. after exposure of steroid to the tissue. No production of estriol from 16-epiestriol was detectable in this time. Considerably more 16-epiestriol was formed from estriol than estriol by the reverse reaction after 90 min. of incubation. 16-Oxo-estradiol-17[beta] appeared in all cases to be the major identified intermediate in the interconversion of 16-epiestriol and estriol.