Salmonella Modulates Vesicular Traffic by Altering Phosphoinositide Metabolism
- 18 June 2004
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 304 (5678) , 1805-1807
- https://doi.org/10.1126/science.1098188
Abstract
Salmonella enterica , the cause of food poisoning and typhoid fever, induces actin cytoskeleton rearrangements and membrane ruffling to gain access into nonphagocytic cells, where it can replicate and avoid innate immune defenses. Here, we found that SopB, a phosphoinositide phosphatase that is delivered into host cells by a type III secretion system, was essential for the establishment of Salmonella 's intracellular replicative niche. SopB mediated the formation of spacious phagosomes following bacterial entry and was responsible for maintaining high levels of phosphatidylinositol-three-phosphate [PtdIns(3)P] in the membrane of the bacteria-containing vacuoles. Absence of SopB caused a significant defect in the maturation of the Salmonella -containing vacuole and impaired bacterial intracellular growth.Keywords
This publication has 35 references indexed in Scilit:
- Vacuole Size Control: Regulation of PtdIns(3,5)P2Levels by the Vacuole-associated Vac14-Fig4 Complex, a PtdIns(3,5)P2-specific PhosphataseMolecular Biology of the Cell, 2004
- Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteriaFEBS Letters, 2003
- Regulation of Fab1 Phosphatidylinositol 3-Phosphate 5-Kinase Pathway by Vac7 Protein and Fig4, a Polyphosphoinositide Phosphatase Family MemberMolecular Biology of the Cell, 2002
- Role of 3-Phosphoinositides in the Maturation of Salmonella-containing Vacuoles within Host CellsPublished by Elsevier ,2002
- SalmonellaInteractions with Host Cells: Type III Secretion at WorkAnnual Review of Cell and Developmental Biology, 2001
- Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificitiesBiochemical Journal, 2000
- Targeting of Salmonella typhimurium to vesicles containing lysosomal membrane glycoproteins bypasses compartments with mannose 6-phosphate receptors.The Journal of cell biology, 1995
- The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteinsMolecular Microbiology, 1994
- Salmonella stimulate macrophage macropinocytosis and persist within spacious phagosomes.The Journal of Experimental Medicine, 1994
- Characterization of the micro‐environment of Salmonella typhimurium–containing vacuoles within MDCK epithelial cellsMolecular Microbiology, 1992