Covalent chromatography. Preparation of fully active papain from dried papaya latex
- 1 July 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 133 (3) , 573-584
- https://doi.org/10.1042/bj1330573
Abstract
No abstract availableKeywords
This publication has 24 references indexed in Scilit:
- Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2′-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiolsBiochemical Journal, 1973
- Isolation by Covalent Affinity Chromatography of the Penicillin-Binding Components from Membranes of Bacillus subtilisProceedings of the National Academy of Sciences, 1972
- The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2′-dipyridyl disulphideBiochemical Journal, 1972
- The reaction of papain with Ellman's reagent [5,5′-dithiobis-(2-nitrobenzoate) dianion]Biochemical Journal, 1972
- A novel reactivity of papain and a convenient active site titration in the presence of other thiolsFEBS Letters, 1970
- Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.1970
- The mechanism of the activation of papainBiochemical and Biophysical Research Communications, 1969
- Kinetics of Papain-Catalyzed Hydrolysis of α-N-Benzoyl-L-arginine Ethyl Ester and α-N-Benzoyl-L-argininamide1Journal of the American Chemical Society, 1965
- The Sulfur Distribution of PapainJournal of Biological Chemistry, 1965
- KINETICS OF PAPAIN ACTION .3. HYDROLYSIS OF BENZOYL-L-ARGININE ETHYL ESTER1958