Dietary and hormonal regulation of the content of acetyl coenzyme A carboxylase-synthesizing polysomes in rat liver.
- 1 July 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (7) , 2304-2307
- https://doi.org/10.1073/pnas.73.7.2304
Abstract
Specific polysomes involved in the synthesis of acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] were identified by the binding of 125I-labeled antiacetyl-CoA carboxylase to rat liver polysomes. The binding is highly specific and occurs through the recognition of the nascent peptide chains on polysomes. The 125I-labeled antibody binding technique demonstrated that the relative content of acetyl-CoA carboxylase-synthesizing polysomes in the liver correlates well with the rate of hepatic synthesis of the enzyme in rats subjected to different dietary conditions as well as in alloxan-diabetic rats with or without insulin treatment.Keywords
This publication has 14 references indexed in Scilit:
- Regulation of synthesis of hepatic fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes.Proceedings of the National Academy of Sciences, 1975
- Acetyl‐Coenzyme‐A Carboxylase in Cultured HepatocytesEuropean Journal of Biochemistry, 1975
- Regulation of Lipogenesis in Animal TissuesCurrent Topics in Cellular Regulation, 1974
- Synthesis of Rat Liver Albumin in a Rabbit Reticulocyte Cell-free Protein-synthesizing SystemJournal of Biological Chemistry, 1973
- Identification and isolation of ovalbumin-synthesizing polysomes. II. Quantification and immunoprecipitation of polysomes.1972
- Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.1972
- Fatty acid synthesis during fat-free refeeding of starved ratsJournal of Lipid Research, 1965
- [On disorders in fatty acid synthesis in hunger and alloxan diabetes. II. Fatty acid synthesis in the liver of alloxan diabetic rats].1963
- [On disorders of fatty acid synthesis in hunger and alloxan diabetes. I. Fatty acid synthesis in the liver of normal and fasting rats].1961
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951