The Transcription of Various Types of Ribonucleic Acid by Hepatocytes Isolated From Rats of Various Ages

Publisher Website
Google Scholar
Abstract
The synthesis of various types of ribonucleic acid (RNA) isolated from 6-to 30-month-old female Fischer F344 rats was compared. The rate of RNA synthesis by freshly prepared hepatocytes was determined by dividing the amount of [3H]orotic acid incorporated into RNA as uridine-5'-monophosphate by the specific activity of the uridine-5'-triphosphate pool. The rate of total RNA synthesis by hepatocytes from 19-month-old rats was 40% less than the rate for hepatocytes from 12-month-old rats. No significant difference in the rate of total RNA synthesis was observed between 19 and 30 months of age. The percentage of [3H]orotic acid incorporated into poly (A)+RNA by 30-month-old rats was approximately 50% less than that observed for hepatocytes isolated from 6-month-old rats. The percentage of [3H]orotic acid incorporated into poly(A)−RNA as ribosomal RNA (38S, 18S, and 5S RNAs) or transfer RNA was similar for 12- and 30-month-old rats. The rate of poly(A)+RNA synthesis by hepatocytes isolated from 30-month-old rats was 65% less than that observed for hepatocytes from 6-month-old rats. In contrast to total RNA synthesis, the rate of poly(A)+ RNA synthesis for the 30-month-old rats was significantly less than the rate for 19-month-old rats.

This publication has 0 references indexed in Scilit: