EFFECT OF NORMAL RAT URINE ON MUCOSAL REGENERATION IN HETEROTOPIC URINARY-BLADDER

  • 1 January 1980
    • journal article
    • research article
    • Vol. 42  (1) , 76-84
Abstract
A heterotopically transplanted urinary bladder (HTB) of the rat with a communicating Ommaya reservoir was developed as a model to evaluate potential bladder carcinogens and the role of urine on the carcinogenesis. Following transplantation the mucosa undergoes ischemic necrosis, but regeneration ensues rapidly within 2 wk. The effect of 2 variants on the natural history of the HTB mucosa was studied immediately following its transplantation: presence of normal rat urine, and the effect of alteration of intraluminal osmolality. Alteration of osmolality had no effect on the regenerative process and the epithelium returned to normal in a few wk. Weekly administration of pooled sterile rat urine into HTB induced simple hyperplasia which persisted throughout the 20 wk study period. The HTBs maintained with pooled rat urine did not attain the normal ultrastructural surface characteristics even at 20 wk posttransplantation. Urine exerts a stimulatory effect on the bladder epithelium when 1st administered to HTB during the active regenerating phase and as long as urinary contact continues.