Anti-Capsular Polysaccharide Antibodies Reduce Nasopharyngeal Colonization byHaemophilus influenzaeType b in Infant Rats

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Abstract
Haemophilus influenzae type b (Hib) conjugate vaccines reduced oropharyngeal carriage of Hib among children in Finland and the United States. To study the mechanism of this reduction, a colonization model in infant rats with passively administered antibodies was developed. Ofthe pups, 94% were colonized 48 hand 64% 7 days after intranasal inoculation with ∼2500 cfu of Hib. Intranasally administered anti-Hib antibodies, including human IgG and both serum and secretory IgA (sIgA) as well as murine monoclonal anti-Hib capsular polysaccharide (PS) of IgG 1 isotype, given simultaneously with, before, or after bacteria, significantly reduced nasopharyngeal colonization by Hib. Hib colonization was also significantly reduced when the antibodies were given intraperitoneally, with a resultant anti-Hib PS serum concentration of ⩾7 µg/mL. Thus, anti-Hib PS antibodies, both sIgA and IgG, can function on the mucosal surface and prevent colonization.

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