Determination of Astragaloside IV in Radix Astragali (Astragalus membranaceus var. monghulicus) Using High-Performance Liquid Chromatography with Evaporative Light-Scattering Detection
Open Access
- 1 November 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 39 (11) , 459-462
- https://doi.org/10.1093/chromsci/39.11.459
Abstract
A reverse-phase high-performance liquid chromatographic method is developed for the determination of astragaloside IV, a characteristic constituent in Radix Astragali. Samples are analyzed by means of a reverse-phase column (Zorbax Eclipse XDB C18) using acetonitrile and water under gradient conditions as the mobile phase for 30 min. An evaporative light-scattering detector is used and set at an evaporating temperature of 43°C with a nebulizing gas (compressed air) pressure of 3.4 bar. The detection limit (signal-tonoise ratio < 5) of astragaloside IV is 40 ng on-column.Keywords
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