Miscibility Phase Diagrams of Giant Vesicles Containing Sphingomyelin
Top Cited Papers
- 13 April 2005
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 94 (14) , 148101
- https://doi.org/10.1103/physrevlett.94.148101
Abstract
Saturated sphingomyelin (SM) lipids are implicated in lipid rafts in cell plasma membranes. Here we use fluorescence microscopy to observe coexisting liquid domains in vesicles containing SM, an unsaturated phosphatidylcholine lipid (either DOPC or POPC), and cholesterol. We note similar phase behavior in a model membrane mixture without SM (), but find no micron-scale liquid domains in membranes of . We delineate the onset of solid phases below the miscibility transition temperature, and detail indirect evidence for a three-phase coexistence of one solid and two liquid phases.
Keywords
This publication has 27 references indexed in Scilit:
- Liquid Domains in Vesicles Investigated by NMR and Fluorescence MicroscopyBiophysical Journal, 2004
- Lipid Dynamics and Domain Formation in Model Membranes Composed of Ternary Mixtures of Unsaturated and Saturated Phosphatidylcholines and CholesterolBiophysical Journal, 2003
- Separation of Liquid Phases in Giant Vesicles of Ternary Mixtures of Phospholipids and CholesterolBiophysical Journal, 2003
- Lipid RaftsCell, 2003
- Fluorescence Energy Transfer Reveals Microdomain Formation at Physiological Temperatures in Lipid Mixtures Modeling the Outer Leaflet of the Plasma MembraneBiophysical Journal, 2003
- Role of cholesterol in lipid raft formation: lessons from lipid model systemsBiochimica et Biophysica Acta (BBA) - Biomembranes, 2003
- Organization in Lipid Membranes Containing CholesterolPhysical Review Letters, 2002
- Characterization of Cholesterol-Sphingomyelin Domains and Their Dynamics in Bilayer MembranesBiophysical Journal, 2001
- Lipid Rafts Reconstituted in Model MembranesBiophysical Journal, 2001
- Functional rafts in cell membranesNature, 1997