Mycobacterium avium subsp. paratuberculosis Strains from Cattle and Sheep Can Be Distinguished by a PCR Test Based on a Novel DNA Sequence Difference
Open Access
- 1 December 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (12) , 4760-4762
- https://doi.org/10.1128/jcm.40.12.4760-4762.2002
Abstract
A DNA sequence differing between sheep and cattle types of Mycobacterium avium subsp. paratuberculosis was identified and used to develop a PCR test. The test unequivocally distinguished all sheep types from cattle types and was negative for a wide range of other strains from the Mycobacterium avium - Mycobacterium intracellulare complex. The test will be useful for epidemiological purposes, particularly in hosts such as deer that can be easily infected with either type.Keywords
This publication has 14 references indexed in Scilit:
- Effect of Commercial-Scale High-Temperature, Short-Time Pasteurization on the Viability ofMycobacterium paratuberculosisin Naturally Infected Cows' MilkApplied and Environmental Microbiology, 2002
- Johne's disease in New Zealand: the past, present and a glimpse into the futureNew Zealand Veterinary Journal, 2002
- Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal SamplesJournal of Clinical Microbiology, 2002
- Mycobacterium aviumsubsp.paratuberculosisin Veterinary MedicineClinical Microbiology Reviews, 2001
- Paratuberculosis Infection of Nonruminant Wildlife in ScotlandJournal of Clinical Microbiology, 2001
- PCR-restriction endonuclease analysis for identification and strain typing ofMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.aviumbased on polymorphisms in IS1311Molecular and Cellular Probes, 1999
- Use of four DNA insertion sequences to characterize strains of theMycobacterium aviumcomplex isolated from animalsMolecular and Cellular Probes, 1997
- Characterization of a Mycobacterium smegmatis gene that confers resistance to phages L5 and D29 when overexpressedMolecular Microbiology, 1996
- Paratuberculosis in Farmed Deer: Case Reports and DNA Characterization of Isolates of Mycobacterium ParatuberculosisJournal of Veterinary Diagnostic Investigation, 1993
- Comparison of polymerase chain reaction tests and faecal culture for detecting Mycobacterium paratuberculosis in bovine faecesVeterinary Microbiology, 1993