Isozyme analysis of the grape (Vitis). I. A practical solution

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Abstract
Isozyme electrophoresis has potential for assisting in cultivar identification of the grape (Vitis). However, past studies have suffered from problems with extraction and resolution of enzymes because of high tannin concentrations in the tissues. In addition, many extraction procedures require expensive, elaborate, and time-consuming protocols. A practical and inexpensive procedure that allows for rapid extraction and the resolution of a large suite of enzymes is presented. Using our procedure, 40 enzymes were tested for their resolvability on numerous gel buffer systems and their usefulness for discriminating cultivars. Fourteen of the enzymes we tested were consistently resolvable and showed variation among cultivars on three gel–electrode buffer systems.

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