EFFECT OF ALKYL CHAIN LENGTH OF STATIONARY PHASE ON RETENTION AND SELECTIVITY IN REVERSED PHASE LIQUID CHROMATOGRAPHY

Abstract
Effect of alkyl chain length of stationary phase on retention and selectivity of various hydrocarbons was found to be strongly dependent on the type of organic solvents in mobile phase in reversed phase liquid chromatography. The results suggest the participation of methanol in the retention of polynuclear aromatic hydrocarbons in stationary phase having long alkyl chains.