Gas chromatographic‐mass spectrometric confirmation of 19‐nortestosterone in the urine of untreated boars — effect of the administration of Laurabolin®

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Abstract
The presence of 17β‐19‐nortestosterone (nandrolone, NT, 17β‐19‐NT) and its epimer 17α‐19‐nortestosterone (epiNT, 17α‐19‐NT) was investigated in the urine of six untreated boars, obtained from experimental farms. The presence of 17β‐19‐nortestosterone was screened by RIA and HPTLC and confirmed by GC‐MS analysis. Additionally, the two epimers (NT and epiNT) were investigated in the urine of a boar (two‐year‐old miniature male pig weighing 50 kg) before and after injection of 100 mg Laurabolin® (nortestosterone laurate, Intervet N.V, Belgium). The isolation of the steroids was based on sample clean‐up with solid phase extraction and subsequent high‐performance liquid chromatography. Both gas chromatographic retention data and mass spectrometric data (selected ion monitoring and full spectrum) were used for detection and identification. The presence of 17β‐19‐nortestosterone in the urine of the boars that were not injected proves the endogenous production of the steroid The absence of the 17α‐epimer in the urine of the injected boar suggests that 17α‐19‐nortestosterone is not a major metabolite of 17β‐19‐ nortestosterone. All correspondence should be addressed to: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Harelbekestraat 72, 9000 Ghent, Belgium.

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